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Wed 20 Jan - Thu 21 Jan 2021
09:00 - 12:30

Venue: Bioinformatics Training Facility - Online LIVE Training (Time Zone = BST (GMT+1) in Summer, GMT in Winter)

Provided by: Bioinformatics


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Next Generation Sequencing Platforms and Bioinformatics Analysis (ONLINE LIVE TRAINING)
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Wed 20 Jan - Thu 21 Jan 2021

Description

PLEASE NOTE The Bioinformatics Team are presently teaching as many courses live online. We aim to simulate the classroom experience as closely as possible, with opportunities for one-to-one discussion with tutors and a focus on interactivity throughout.

Day 1 will introduce you to next generation sequencing technologies (NGS) and how they work, providers, common bioinformatics workflows, standardised file types, quality control. This session will include an introduction to Galaxy. Galaxy is an open, web-based platform for data-intensive life science research that enables non-bioinformaticians to create, run, tune, and share their own bioinformatic analyses.

Day 2 will be hands-on practicals on using Galaxy to explore sequencing quality control, before and after removal of low quality samples. This forms the core of all NGS analyses and this day will conclude with how this data pipes into gene expression studies, variant calling and genome assemblies.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

Target audience
  • Biologists, wet-lab scientists, bioinformatics and other life scientists planning to work with next-generation sequencing data.
  • Graduate students, Postdocs and Staff members from the University of Cambridge, Affiliated Institutions and other external Institutions or individuals
  • Please be aware that these courses are only free for registered University of Cambridge students. All other participants will be charged a registration fee in some form. Registration fees and further details regarding the charging policy are available here.
  • Further details regarding eligibility criteria are available here
Prerequisites
  • An undergraduate level understanding of molecular biology is required.
  • No prior experience with using Galaxy is required.
Sessions

Number of sessions: 2

# Date Time Venue Trainer
1 Wed 20 Jan   09:00 - 12:30 09:00 - 12:30 Bioinformatics Training Facility - Online LIVE Training (Time Zone = BST (GMT+1) in Summer, GMT in Winter) Gareth Price
2 Thu 21 Jan   09:00 - 12:30 09:00 - 12:30 Bioinformatics Training Facility - Online LIVE Training (Time Zone = BST (GMT+1) in Summer, GMT in Winter) Gareth Price
Topics covered

Bioinformatics,

Objectives

After this course you should have an understanding of:

  • Current next-generation sequencing providers
  • Selecting the most appropriate technology for a specific biology question
  • How to use check file type
  • Import of data into Galaxy and perform Quality Control
Aims

During this course you will learn about:

  • Introduction to NGS technology
  • NGS providers and how their technology works
  • Common bioinformatics workflows
  • Standardised file types
  • Sequence data quality controls
  • Introduction to Galaxy
Format

Presentation, demonstration and hands-on practicals

Timetable

This is subject to change in line with the online training schedule.

Day 1 Topics
9:00 - 10:00 Introduction to NGS (theory): Learn about the terminologies, platforms, pipeline overview
10:00 - 10:30 A short introduction to Galaxy
10:30 - 10:45 Break
10:45 - 11:45 Q and A
Day 2 Topics
9:00 - 9:30 Introduction to NGS (theory): How data generation impacts on data quality
9:30 - 10:30 Hands on Quality Control practical
10:30 - 10:45 Break
10:45 - 11:45 Continuing Hands On with more advanced Workflow training
11:45 - 12:30 Q and A
Registration Fees
  • Free for registered University of Cambridge students
  • £ 50/day for all University of Cambridge staff, including postdocs, temporary visitors (students and researchers) and participants from Affiliated Institutions. Please note that these charges are recovered by us at the Institutional level
  • It remains the participant's responsibility to acquire prior approval from the relevant group leader, line manager or budget holder to attend the course. It is requested that people booking only do so with the agreement of the relevant party as costs will be charged back to your Lab Head or Group Supervisor.
  • £ 50/day for all other academic participants from external Institutions and charitable organizations. These charges must be paid at registration
  • £ 100/day for all Industry participants. These charges must be paid at registration
  • Further details regarding the charging policy are available here
Duration

Two half day sessions

Frequency

Once per year

Related

Theme
Specialized Training

Booking / availability