Introduction to Galaxy for Next Generation Sequencing Analysis (ONLINE LIVE TRAINING)

Day 1 will introduce you to next generation sequencing technologies (NGS) and how they work, providers, common bioinformatics workflows, standardised file types, quality control. This session will include an introduction to Galaxy. Galaxy is an open, web-based platform for data-intensive life science research that enables non-bioinformaticians to create, run, tune, and share their own bioinformatic analyses.

Day 2 will be hands-on practicals on using Galaxy to explore sequencing quality control, before and after removal of low quality samples. This forms the core of all NGS analyses and this day will conclude with how this data pipes into gene expression studies, variant calling and genome assemblies.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

• Biologists, wet-lab scientists, bioinformatics and other life scientists planning to work with next-generation sequencing data.
• Graduate students, Postdocs and Staff members from the University of Cambridge, Affiliated Institutions and other external Institutions or individuals
• Please be aware that these courses are only free for registered University of Cambridge students. All other participants will be charged a registration fee in some form. Registration fees and further details regarding the charging policy are available here.
• Further details regarding eligibility criteria are available here

• An undergraduate level understanding of molecular biology is required.
• No prior experience with using Galaxy is required.

Number of sessions: 2

Bioinformatics,

After this course you should have an understanding of:

• Current next-generation sequencing providers
• Selecting the most appropriate technology for a specific biology question
• How to use check file type
• Import of data into Galaxy and perform Quality Control

During this course you will learn about:

• Introduction to NGS technology
• NGS providers and how their technology works
• Common bioinformatics workflows
• Standardised file types
• Sequence data quality controls
• Introduction to Galaxy

Presentation, demonstration and hands-on practicals

This is subject to change in line with the online training schedule.

• Free for registered University of Cambridge students
• £ 50/day for all University of Cambridge staff, including postdocs, temporary visitors (students and researchers) and participants from Affiliated Institutions. Please note that these charges are recovered by us at the Institutional level
• It remains the participant's responsibility to acquire prior approval from the relevant group leader, line manager or budget holder to attend the course. It is requested that people booking only do so with the agreement of the relevant party as costs will be charged back to your Lab Head or Group Supervisor.
• £ 50/day for all other academic participants from external Institutions and charitable organizations. These charges must be paid at registration
• £ 100/day for all Industry participants. These charges must be paid at registration
• Further details regarding the charging policy are available here

Two half day sessions

A few times per year

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